Spatial biology enables the interrogation of tissue composition at a single cell level with the preservation of spatial context, which opens new avenues for tumor microenvironment (TME) studies [1]. Biomarkers’ composition of the tissue can be interrogated with hyperplexed immunofluorescence, wherein an imaging detection is performed for each marker on the same slide. The COMET™ platform performs sequential immunofluorescence (seqIF ™) and enables full automation of such workflow, where: up to 40 biomarkers can be detected with full automation from staining to data acquisition. The resulting hyperplex images are rich sources of data about the specimens. To extract information from such a dataset, Phenoplex™ (Oncotopix® Discovery, Visiopharm) has a dedicated workflow for image analysis that delivers single-cell phenotypic information and biodistribution, providing access to the spatial composition of the TME.

David Mason², Joanna Kowal¹, James Mansfield², Pedro Machado Almeida¹, Regan Baird², Benjamin Pelz¹, Fabian Schneider², Diego G. Dupouy¹

1. Lunaphore Technologies S.A., Tolochenaz, Switzerland
2. Visiopharm A/S, Hørsholm, Denmark