Imaging Mass Cytometry™ (IMC™) is a powerful tool for the study of complex cellular interactions in the tumor microenvironment (TME) and in the discovery of biomarkers that can predict disease outcome or response to therapy. The Hyperion™ Imaging System (Standard BioTools™) utilizes CyTOF® technology to simultaneously assess 40-plus protein markers at subcellular resolution without spectral overlap or background autofluorescence, thus providing unprecedented insight into the organization and function of the TME. However, despite the advances in staining and imaging methods, developments in analysis software have not kept pace, as we lack a complete, user-defined workflow in a single software package for analysis of high-plex imaging data. Here we present a comprehensive workflow using the Visiopharm® Phenoplex™ platform, designed specifically for high-plex IMC image analysis.

Smriti Kala¹, Brenna O’Neill², Rasmus Lyngby², Rasmus Sorensen², Andrew Quong¹, Sam Lim¹, Clinton Hupple¹, Nina Lane¹, Jeppe Thagaard², Johan Dore Hansen², James Mansfield², Fabian Schneider², Michelle Macpherson¹

1. Standard BioTools Canada Inc., Markham, Ontario, Canada
2. Visiopharm A/S, Hørsholm, Denmark