Imaging Mass Cytometry™ (IMC™) is a powerful tool for the study of complex cellular interactions in the tumor microenvironment (TME) and in the discovery of biomarkers that can predict disease outcome or response to therapy. The Hyperion™ Imaging System (Standard BioTools™) utilizes CyTOF® technology to simultaneously assess 40-plus protein markers at subcellular resolution without spectral overlap or background autofluorescence, thus providing unprecedented insight into the organization and function of the TME. However, despite the advances in staining and imaging methods, developments in analysis software have not kept pace, as we lack a complete, user-defined workflow in a single software package for analysis of high-plex imaging data. Here we present a comprehensive workflow using the Visiopharm® Phenoplex™ platform, designed specifically for high-plex IMC image analysis.
Smriti Kala1, Brenna O’Neill2, Rasmus Lyngby2, Rasmus Sorensen2, Andrew Quong1, Sam Lim1, Clinton Hupple1, Nina Lane1, Jeppe Thagaard2, Johan Dore Hansen2, James Mansfield2, Fabian Schneider2, Michelle Macpherson1
- Standard BioTools Canada Inc., Markham, Ontario, Canada
- Visiopharm A/S, Hørsholm, Denmark