Tissue stained with DAPI
#10169
Nuclei Detection, AI (Fluorescence)
With just a few clicks, you can obtain clearly segmented nuclei in your fluorescence images (whole slide or TMA) with this Quickstart APP. Nuclei can be difficult to detect accurately and precisely across different images using traditional image analysis with feature engineering. This APP utilizes artificial intelligence (AI) for automatic nuclear segmentation and the data is compatible with the Phenoplex™ Guided Workflow (for Phenoplex™) and the Data Exploration and QC tool (Discovery and Phenoplex™).
All Quickstart APPs can be sequenced as part of a larger image analysis pipeline, with the batch analysis feature, which enables walk-away analysis. They can also be customized and serve to create downstream analysis stems.
Nuclear segmentation in DAPI stained tissue. The APP can be set to count the total number of nuclei.
Detailed view of tissue stained with DAPI.
Detailed view of nuclear segmentation in DAPI stained tissue. The APP can be set to count the total number of nuclei.
Details
Quantitative Output variables
- Total nuclei (#)
- Biomarker intensity for each nucleus
Workflow
After loading your image follow this step:
Step 1: Open the “10169 – Nuclei Detection, AI (Fluorescence)” APP
Step 2: Select a region of interest or skip to the next step
Step 3: Click “Run APP” or perform batch analysis to queue this APP and keep working on other images
