Unprocessed multiplex GeoMx® DSP image.
#10193
GeoMx® DSP Cell Detection
This Quickstart APP segments cells in images acquired through the GeoMx® Digital Spatial Profiling method by Bruker. After you have highlighted your region of interest, this APP will quickly and accurately segment the cells for further downstream analyses.
As with all of our Quickstart APPs, your image analysis can be scaled by queueing APPs to run in the background on an unlimited number of images—this is possible with the “batch analysis” feature available on both Discovery and Phenoplex™.
Working with multimodal data sets or TMAs? No problem. Run this APP after using the Tissuealign™ feature to align your images at the cellular level, or the Tissuearray™ feature to de-array your tissue cores.
After analysis is complete, you may explore your results with the Phenoplex™ Guided Workflow or the Data Exploration and QC tool (available on Discovery and Phenoplex™).
Segmented cells in the processed image. Results also include biomarker intensity in the nuclei, cell bodies, and entire cell.
Another area in the same image with fewer cells before processing with this APP.
This APP was able to accurately and precisely segment cells in this region.
Details
Quantitative Output variables
- Total cell count
- Biomarker intensity for the entire cell, nuclei, and cell bodies
Workflow
After loading your image, follow these steps.
Step 1: Open the “10193 – GeoMx® DSP Cell Detection” APP.
Step 2: Select a region of interest or skip to next step.
Step 3: Click “Run APP” or perform batch analysis to queue this APP and keep working on other images
