PCK VDS, Tumor Detection PCK VDS, Tumor Detection

Process APP

Revert

#10165

Ancillary APP

PCK VDS, Tumor Detection

Developed for tumor detection in PCK stained breast tissue

This APP has been developed to improve quantification of ER positive cells within breast carcinoma, by automatically identifying tumor areas. The APP uses the Virtual Double Staining (VDS) technique, which enables automated and robust detection of tumor regions.

Two serial sections stained for ER and Pancytokeratin (PCK), respectively, must be used in this APP. Tumor regions are identified automatically on the PCK stained slides and the outlined tumor region are overlaid on the ER stained slide, thus automatically identifying tumor regions.

Ancillary APP

Figure 1

Figure 1

The original TMA-core stained with PCK.

Figure 2

Figure 2

The original TMA-core stained with ER.

Figure 3

Figure 3

Tissue alignment. The PCK and ER TMA-cores are aligned, one on top of the other.

Figure 4

Figure 4

Part of tissue stained with PCK.

Figure 5

Figure 5

The tumor region is segmented in the PCK tissue and outlined with a ROI. The ROI obtained from the PCK tissue is superimposed on the aligned ER issue (not seen here). The ROI only outlines tumor regions. Tissue that is not tumor and the background are excluded.

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Details

Quantitative Output variables

The output consists of one or more ROIs, outlining the tumor areas. The output variable obtained from this protocol is:

  • Tumor Area: The total area of the tumor regions measured in [µm²]

Workflow

Step 1: Load and run the APP “10165 – PCK VDS, Tumor Detection” in a region on the image of interest

Methods

To employ the VDS approach for ER, two serial sections must be stained for ER and PCK, respectively. This APP works as a three-step process as described below.

First, alignment of the two serial sections (see FIGURE 3). The alignment is done both on a large scale, and on a finer detailed level, to get the best possible match of the two tissue sections.

Second, the tumor areas are automatically detected from the PCK slide and outlined as regions of interest (ROIs) (see FIGURE 4 and 5). The ROIs are then superimposed on the aligned ER tissue slide to outline the tumor region for subsequent analysis limited to the inside of the tumor regions.

Staining Protocol

There is no staining protocol available.

Additional information

This APP has been developed in cooperation with Professor Mogens Vyberg from NordiQC and Aalborg University Hospital Denmark.

Keywords

VDS, Virtual Double Staining, breast carcinoma, immunohistochemistry, breast cancer, quantitative, digital pathology, image analysis, PCK, Cytokeratin, TMA

References

LITERATURE
1. Hammond, E. H. et. al. American Society of Clinical Oncology/College of American Pathologists Guideline Recommendations for Immunohistochemical Testing of Estrogen and Progesterone Receptors in Breast Cancer, Archives of pathology & laboratory medicine 2010, 134 (7), e48-72, DOI.

2. Kårsnas, A., Dahl, A. L., Larsen, R. Learning histopathological patterns, J. P. Inform. 2011, 2 (2), 12, DOI.

3. Jung, C., Changick, K. Segmenting Clustered Nuclei Using H-minima Transform-Based Marker Extraction and Contour Parameterization, IEEE Transactions on Biomedical Engineering 2010, 57 (10), 2600-2604, DOI.

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