
The auxiliary APP “01 ROI detect” automatically outlines the relevant tissue on slide.
#10085
Microscopic colitis is a type of inflammation of the colon that causes non-bloody watery diarrhea and most often impacts middle-aged females. The condition can be distinguished into two types, lymphocytic and collagenous. The difference between the two types are minor; both are characterized by an increase in lymphocytes with collagenous colitis also having a thickened subepithelial collagen layer.
This APP is addressing lymphocytic colitis.
By quantifying the T-lymphocytes targeted by the CD3 marker in different tissue compartments, a diagnosis can be determined.
Auxiliary APPs
ROI detect:
APP: “01 ROI detect”
This APP can be used for automatic and precise detection of tissue present in the image, thereby limiting the analysis to only occur within relevant tissue areas and not on the entire image.
Quantitative Output variables
The output variables obtained from this protocol are:
Methods
The APP detects the tissue regions and separates the intestinal glands and tissue border from the remaining tissue via edge detection based on Hematoxylin deconvolution. The brown CD3 positive stained and blue negative stained areas are then quantified and calculated within the three tissue regions.
Staining Protocol
There is no staining protocol available.
Keywords
Colon, colorectal, colitis, CD3, lymphocytic, collagenous, human, digital pathology, image analysis, quantitative.
References
LITERATURE
There are no references available.